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微信小章| 产品名称： | pINT-800 pCAT int1 |
|---|---|
| 商品货号： | TS135333 |
| Designations： | pINT-800 pCAT int1 |
| Species： | Homo sapiens, human |
| Depositors： | RA Gravel |
| Applications： | Codominant segregation of the MspI RFLP was observed in 3 Caucasian families (9 individuals). Enzyme(s) not detecting polymorphism: AluI, ApaI, BamHI, BglI, BglII, Bsp1286, DraI, EcoRI, HaeIII, HincII, HinfI, PstI, PvuII, ScaI, SphI, SstI, StuI, XbaI (tested against 7 unrelated North American Caucasians). |
| Vector： | Construct size (kb): 3.799999952316284 |
| Insert： | DNA: genomic Insert lengths(kb): 0.800000011920929 Tissue: fetal liver Gene product: catalase CAT Alleles: C1, A1, E1, E2, A2, C2 |
| Insert Size (kb)： | 0.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): Sal--uncut; BamHI/SalI--3.8; SalI/EcoRI--3.8; HindIII/EcoRI--3.0, 0.5, 0.3; AccI/AvaI--3.8; EcoRI/PstI--2.8, 0.9; EcoRI--3.8. Codominant segregation of the MspI RFLP was observed in 3 Caucasian families (9 individuals). The insert contains a portion of intron 1 which separates the exons coding for amino acids 1-21 and 22-79 of the gene. Codominant segregation of the TaqI RFLP was demonstrated in 5 families of 26 individuals. Enzyme(s) not detecting polymorphism: AluI, ApaI, BamHI, BglI, BglII, Bsp1286, DraI, EcoRI, HaeIII, HincII, HinfI, PstI, PvuII, ScaI, SphI, SstI, StuI, XbaI (tested against 7 unrelated North American Caucasians). |
| References： | Wadey RB, Cowell JK. A MspI RFLP associated with the human catalase gene. Nucleic Acids Res. 17: 3332, 1989. PubMed: 2471153 Schroeder WT, et al. Nonrandom loss of maternal chromosome 11 alleles in Wilms tumors. Am. J. Hum. Genet. 40: 413-420, 1987. PubMed: 2883892 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Quan F, et al. An RFLP associated with the human catalase gene. Nucleic Acids Res. 13: 8288, 1985. PubMed: 2933636 Quan F, et al. Isolation and characterization of the human catalase gene. Nucleic Acids Res. 14: 5321-5335, 1986. PubMed: 3755525 |