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| 产品名称： | BP-TIE2-R2-1 |
|---|---|
| 商品货号： | TS135352 |
| Designations： | BP-TIE2-R2-1 |
| Species： | Homo sapiens, human |
| Depositors： | R Haronen |
| Vector： | Construct size (kb): 8.899999618530273 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBacPAK8 Intact vector size: 5.538 Type of vector: phagemid Vector end: XhoI Vector end: EagI Cloning sites: BstXI mLuI EcoRV HindIII DraIII ScaI Polylinker sites: BamHI Sse8387I PstI StuI XhoI BstBI XbaI BglII Asp718I KpnI Ecl136II SacI EcoRI XmaI SmaI EagI NotI PacI Features (with orientation and position when available): promoter: polyhedrin, -> MCS: multiple cloning site, -> stop codons: stop codons, -> marker(s): ampR replicon: M13 replicon: pMB1 coding sequence: AcMNPV Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: TEK Gene name: TEK tyrosine kinase, endothelial, mutated ORFs seq. position: DNA Seq. Acc.: L06139 () Contains complete coding sequence?: Y Chromosome: 9; Localization: 9 p21 Type of DNA: cDNA Insert 5 end: XhoI Insert 3 end: EagI Insert size (kb): 3.4 Cross references: GDB probe: GDB:344185 Insert lengths(kb): 3.400000095367432 Gene product: TEK tyrosine kinase, endothelial, mutated TEK |
| Insert Size (kb)： | 3.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.7, 1.2; NcoI/XhoI--5.5, 2.8, 0.7. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.9, 1.4; SmaI--2.9. TS135352 contains wild-type receptor tyrosine kinase TIE2 and ATCC 87507 contains a mutant gene that has a point mutation at position 2545 from C to T, resulting in an R849W substitution. |
| References： | Vikkula M, et al. Vascular dysmorphogenesis caused by and activating mutation in the receptor tyrosine kinase TIE2. Cell 87: 1181-1190, 1996. PubMed: 8980225 |