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| 产品名称： | pdBPV-1(142-6) |
|---|---|
| 商品货号： | TS135393 |
| Designations： | pdBPV-1(142-6) |
| Depositors： | PM Howley |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 10.5900001525878900 Vector: pdBPV-1(142-6) (plasmid) Construction: pML2d, pBPV-1(8-2) Marker(s):ampR Construct size (kb): 10.59000015258789 Features: marker(s): ampR replicon: pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.0, 2.6; EcoRI--5.7, 5.0; HindIII--10.5; SalI--10.5; HincII--3.7, 3.3, 1.9, 1.05, 0.85. A shuttle vector which replicates as a stable, nonintegrated, multicopy plasmid in transformed mouse cells and in Escherichia coli. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sarver N, et al. Transformation and replication in mouse cells of a bovine papillomavirus-pML2 plasmid vector that can be rescued in bacteria. Proc. Natl. Acad. Sci. USA 79: 7147-7151, 1982. PubMed: 6296820 |
| Shipped： | freeze-dried |