产品名称: | pHAFP9.9 |
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商品货号: | TS135471 |
Designations: | pHAFP9.9 |
GenBank Number: | M16110 |
Species: | Homo sapiens, human |
Depositors: | A Dugaiczyk |
Vector: | Construct size (kb): 14.30000019073486 |
Insert: | DNA: genomic Insert lengths(kb): 9.899999618530273 Tissue: fetal liver Gene product: alpha-fetoprotein( alpha-1-fetoprotein, alpha-foetoprotein, alpha-fetoglobulin) AFP Alleles: B1, B2, C1, C2, D1, D2 |
Insert Size (kb): | 9.900 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 4.6; EcoRI--10.2, 4.4; PstI--4.3, 4.0, 2.5 (doublet), 0.9; SalI--14.0; HindIII--7.0 (doublet). The insert contains the following restriction sites (nt position according to the published sequence): PstI--1843, 2786, 5318, 9763; HindIII--2775; BamHI--5462. This is the EcoRI fragment extending from nt 1 to 9906 of the published sequence subcloned from lambda33. Contains exons 1-7 and an Alu repeat. The polymorphic site is at nt 9494 of the published sequence. |
References: | Gibbs PE, et al. Structure, polymorphism, and novel repeated DNA elements revealed by a complete sequence of the human alpha-fetoprotein gene. Biochemistry 26: 1332-1343, 1987. PubMed: 2436661 |