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| 产品名称： | pSE1111 |
|---|---|
| 商品货号： | TS135528 |
| Designations： | pSE1111 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | SJ Elledge |
| Applications： | in another host, produces protein |
| Vector： | Construct size (kb): 8.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pACT Intact vector size: 7.650 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BglII BamHI XhoI Polylinker sites: BglII XhoI BamHI EcoRI BglII Other unique sites: XbaI HapI ClaI EcoRV Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): replicon: 2 micron marker(s): LEU2, -> terminator: ADH MCS: BglII XhoI ... BglII other: GAL4 activation domain promoter for expression: ADH, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: SNF4 Contains complete coding sequence?: U Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 0.9 Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: SNF4 |
| Insert Size (kb)： | 0.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 87014 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.2, 0.9; EcoRI--3.7, 3.0, 0.5; BglII--7.0, 0.94. pSE1111 (TS135528) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems. Expresses SNF4 fused to the GAL4 activation domain. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581 Stephen J Elledge, personal communication |