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| 产品名称： | pRS416 |
|---|---|
| 商品货号： | TS135567 |
| Designations： | pRS416 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli DH5alpha |
| Vector Information： | Size (kb): 4.898 DESCRIPTION OF VECTOR: Intact vector size: 4.898 Type of vector: phagemid Cloning sites: KpnI ApaI XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI EagI NotI SacII SacI NaeI Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII Construction: pBluescriptII KS+ Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): URA3, ->, 417-1219 replicon: f1, insert detection: lacZ, promoter for in vitro transcription: T7, ->, 1977-1996 MCS: KpnI...SacI, ->, 2004-2111 promoter for in vitro transcription: T3, promoter: lac, replicon: pMB1, 2566-2566 marker(s): ampR, centromere: CEN6, 4326-4442 replicon: ARSH4, 4443-4806 Vector: pRS416 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):URA3,ampR Construct size (kb): 4.898 Features: insert detection: lacZ marker(s): URA3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: KpnI...SacI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuII--4.45, 0.45; BglI--3.2, 1.7; PstI--3.25, 1.65. A YC-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Related Products： | component of:ATCC 87534 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |