微信小陈
微信小章| 产品名称： | humfact7 |
|---|---|
| 商品货号： | TS135573 |
| Designations： | humfact7 |
| GenBank Number： | J02933 |
| Species： | Homo sapiens, human |
| Depositors： | PJ OHara |
| Vector： | Construct size (kb): 5.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: hepatocellular carcinoma Hep G2 cell line Gene product: coagulation factor VII (serum prothrombin conversion accelerator) F7 |
| Insert Size (kb)： | 2.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The following enzymes cut the insert once (nt from 5 end, short leader): BssHII-139 MluI-147 XmaIII-167 BglII-228 ScaI-443 Eco47III-470 XbaI-562 NcoI-635 BclI-687 SstII-714 SplI-840 Asp718-1209 KpnI-1213 ApaLI-1996 NsiI-2246. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.6; SacI--3.5, 1.5, 0.45; EcoRI/SacI/XhoI--2.9, 1.5, 0.7, 0.45; BglI--4.3, 1.2. The insert includes the entire coding sequence of the gene. There are two alternate cDNAs for F7, one encoding a leader of 60 amino acids and one a leader of 38 amino acids. This clone encodes the short leader. The depositor indicates the polymorphism has allele lengths that differ by only a few nucleotides. It would be difficult to detect this polymorphism by means of a Southern blot of the genomic digest. |
| References： | OHara PJ, Grant FJ. The human factor VII gene is polymorphic due to variation in repeat copy number in a minisatellite. Gene 66: 147-158, 1988. PubMed: 2970988 Hagen FS, et al. Characterization of a cDNA coding for human factor VII. Proc. Natl. Acad. Sci. USA 83: 2412-2416, 1986. PubMed: 3486420 Huang Q, et al. Substrate recognition by tissue factor-factor VIIa. J. Biol. Chem. 271: 21752-21757, 1996. PubMed: 8702971 OHara PJ, et al. Nucleotide sequence of the gene coding for human factor VII, a vitamin K-dependent protein participating in blood coagulation. Proc. Natl. Acad. Sci. USA 84: 5158-5162, 1987. PubMed: 3037537 Patrick J OHara, personal communication |