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| 产品名称： | YCpIF1 |
|---|---|
| 商品货号： | TS135585 |
| Designations： | YCpIF1 |
| Depositors： | RW Davis, PK Foreman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.5000000000000000 Vector: YCpIF1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pUN105 Marker(s):LEU2,ampR Construct size (kb): 7.5 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GAL1 replicon: ARS1 replicon: pMB1 MCS: SacI...SalI centromere: CEN4 |
| Applications： | YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--7.5; BamHI--7.5; EcoRI--4.3, 3.5. One of several S. cerevisiae shuttle expression vectors (TS135585-87092) differing in selection marker, reading frame and orientation of the promoter. Vectors YCpIF1, YCpIF2 and YCpIF3 (TS135585-87080) differ only in the reading frame of the multiple cloning site. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907 |
| Related Products： | component of:ATCC 87030 |
| Shipped： | freeze-dried |