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| 产品名称： | Canid herpesvirus 1 |
|---|---|
| 商品货号： | TS135729 |
| Classification： | Herpesviridae, Varicellovirus |
| Deposited As： | Canine herpesvirus |
| Agent： | Canid herpesvirus 1 |
| Strain： | D 004 |
| Common Name： | Canine herpesvirus |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Respiratory tract of a dog |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | No cross-neutralization with other herpesviruses. Can only be propagated in cells of canine origin. Purified 1 time by terminal dilution, and 3 times by plaque passage. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： |
MDCK cells (ATCC® CCL-34™)
|
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | LN Binn, Division of Veterinary Medicine, Walter Reed Army Institute of Research |
| Source： | Respiratory tract of a dog |
| References： | Binn LN, et al. Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. Exp. Biol. Med. 126: 140-145, 1967. PubMed: 4294430 |
| Cross References： | Nucleotide (GenBank) : U63459 Canine herpesvirus DNA polymerase gene, partial cds. |