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| 产品名称： | AA35 |
|---|---|
| 商品货号： | TS135760 |
| Designations： | AA35 |
| GenBank Number： | L05929 M91388 |
| Species： | Homo sapiens, human |
| Depositors： | A Swaroop |
| Applications： | contains sequence DNA Segment contains sequence SEC13 (S. cerevisiae) - like 1 |
| Vector： | Construct size (kb): 4.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript KS- Intact vector size: 2.964 Type of vector: phagemid Vector end: EcoRI Vector end: HindIII Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI Polylinker sites: SEE COMMENTS Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, T3, T7 replicon: pMB1, f1 terminator: none Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SEC13L1 Other gene symbols: D3S1231E Gene name: SEC13 (S. cerevisiae) - like 1 Contains complete coding sequence?: Y Chromosome: 3; Localization: 3 p25-p24 Tissue: retinal pigment epithelial cell line Type of DNA: cDNA Insert 5 end: Modification: EcoRI Insert 3 end: Modification: HindIII Insert size (kb): 1.322 Cross references: DNA Seq. Acc.: L05929 Nucleotides 45-405 of the insert correspond to nucleotides 87-447 of L05929 with 61% similarity. DNA Seq. Acc.: M91388 Nucleotides 1322-1085 of the insert correspond to nucleotides 1-238 of M91388 with 100% identity. Insert lengths(kb): 1.322000026702881 Tissue: retinal pigment epithelial cell line Gene product: SEC13 (S. cerevisiae) - like 1 SEC13L1 Target Gene: DNA Segment, SEC13 (S. cerevisiae) - like 1 |
| Insert Size (kb)： | 1.322 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 1.3; PvuII--2.5, 0.7, 0.5, 0.45, 0.2; SacI--3.8, 0.5; SalI--3.1, 1.2; SphI--4.3. The insert contains the following restriction sites (approximate kb from the 5 end): BspHI--0.18; HincII--0.16; PvuII--0.48, 0.71, 1.17; SacI--0.38; SalI--0.16; SphI--0.31. Detects a related sequence on human chromosome 18 q11.2-q12. |
| References： | Swaroop A, et al. Molecular characterization of a novel human gene, SEC13R, related to the yeast secretory pathway gene SEC13, and mapping to a conserved linkage group on human chromosome 3p24-p25 and mouse chromosome 6. Hum. Mol. Genet. 3: 1281-1286, 1994. PubMed: 7987303 |