产品名称: | pHF12-32 DOSLC9, p12-32 |
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商品货号: | TS135782 |
Designations: | pHF12-32 DOSLC9, p12-32 |
Species: | Homo sapiens, human |
Depositors: | RL White |
Vector: | Construct size (kb): 4.900000095367432 |
Insert: | DNA: genomic Insert lengths(kb): 0.5400000214576721 Gene product: DNA Segment, single copy D3S2 Alleles: A1, A2 |
Insert Size (kb): | 0.540 |
Media: | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--4.9; HindIII--4.9; SalI--4.9; EcoRI/HindIII--3.45, 1.55; HindIII/SalI--3.6, 1.2. Detects loss of heterozygosity in renal cell carcinoma. Enzyme(s) not detecting polymorphism: MboI, TaqI, EcoRI, HindIII, BamHI, BglII, BclI, HincII, PstI, NciI, SstI, XmaI, HaeIII, BstNI, BstEII, AccI, StuI, RsaI, EcoRV, AluI, SinI, DdeI, NaeI. |
References: | Barker D, et al. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 36: 131-138, 1984. PubMed: 6198090 Naylor SL, et al. DNA polymorphic loci mapped to human chromosome 3, 5, 9, 11, 17, 18, and 22. Proc. Natl. Acad. Sci. USA 81: 2447-2451, 1984. PubMed: 6585809 Zbar B, et al. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. Nature 327: 721-724, 1987. PubMed: 2885753 Kovacs G, et al. Consistent chromosome 3p deletion and loss of heterozygosity in renal cell carcinoma. Proc. Natl. Acad. Sci. USA 85: 1571-1575, 1988. PubMed: 2894030 |