宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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pHF12-32 [DOSLC9, p12-32]

货号 TS135782
中文名称 null
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产品名称: pHF12-32 DOSLC9, p12-32
商品货号: TS135782
Designations: pHF12-32 DOSLC9, p12-32
Species: Homo sapiens, human
Depositors: RL White
Vector:
Construct size (kb): 4.900000095367432
Insert:
DNA: genomic
Insert lengths(kb): 0.5400000214576721
Gene product: DNA Segment, single copy D3S2
Alleles: A1, A2
Insert Size (kb): 0.540
Media: ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: freeze-dried
Comments:
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--4.9; HindIII--4.9; SalI--4.9; EcoRI/HindIII--3.45, 1.55; HindIII/SalI--3.6, 1.2.
Detects loss of heterozygosity in renal cell carcinoma.
Enzyme(s) not detecting polymorphism: MboI, TaqI, EcoRI, HindIII, BamHI, BglII, BclI, HincII, PstI, NciI, SstI, XmaI, HaeIII, BstNI, BstEII, AccI, StuI, RsaI, EcoRV, AluI, SinI, DdeI, NaeI.
References:

Barker D, et al. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 36: 131-138, 1984. PubMed: 6198090

Naylor SL, et al. DNA polymorphic loci mapped to human chromosome 3, 5, 9, 11, 17, 18, and 22. Proc. Natl. Acad. Sci. USA 81: 2447-2451, 1984. PubMed: 6585809

Zbar B, et al. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. Nature 327: 721-724, 1987. PubMed: 2885753

Kovacs G, et al. Consistent chromosome 3p deletion and loss of heterozygosity in renal cell carcinoma. Proc. Natl. Acad. Sci. USA 85: 1571-1575, 1988. PubMed: 2894030