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| 产品名称： | Simian virus 40 |
|---|---|
| 商品货号： | TS135820 |
| Designations： | pUCSV-VA45-54-2Ea molecular clone of Simian virus 40, SV-40 |
| GenBank Number： | AF156105 |
| Species： | Simian virus 40, Simian virus 40 |
| Depositors： | JA Lednicky, JS Butel |
| Applications： | contains sequence |
| Vector： | Construct size (kb): 7.949999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 5.263999938964844 Tissue: uninoculated grivet monkey cells Gene product: SV-VA45-54-2Ea |
| Insert Size (kb)： | 5.264 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains following restriction sites (from 5end): BamHI-0.771,7.179; EcoRI-2.686,5.264; KpnI-3.786,4.164; SalI-7.950; SfiI-7.950; XhoI-uncut. Plasmid size: 7950bp (2686bp pUC-19vector+ 5264bp SV40-VA45-54-2 insert). This variant of SV-40-VA45-54 has an non-archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-2Ea DNA into ATCC CV-1 cells by the contributor, followed by DNA sequence analysis of the virus stock. |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047 Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955 |