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| 产品名称： | p309 |
|---|---|
| 商品货号： | TS135828 |
| Designations： | p309 |
| Depositors： | JC Lang |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0999999046325680 Vector: p309 (plasmid) Promoters: Promoter lac Construction: pUC18, aph, MSV promoter and enhancer Marker(s):G418R,ampR Construct size (kb): 5.099999904632568 Features: insert detection: lacZ marker(s): ampR, G418R promoter: lac, MSV replicon: pMB1 enhancer: MSV |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | XbaI, SmaI, SstI and EcoRI appear in both the polylinker and the aph cassette. Restriction digests of the clone give the following sizes (kb): HindIII--4.8; EcoRI--4.7, 0.2. An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of murine sarcoma virus promoter and enhancer. The PvuII at position 628 of pUC18 was changed to BglII. A cassette containing the aph gene under control of murine sarcoma virus promoter and enhancer was inserted at the BglII site. Aph is transcribed in the opposite direction of lacZ. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Lang JC, et al. pUC vectors capable of conferring neomycin resistance of eukaryotic cells. Nucleic Acids Res. 16: 7736, 1988. PubMed: 2842735 |
| Shipped： | freeze-dried |