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| 产品名称： | Human adenovirus 4 |
|---|---|
| 商品货号： | TS135857 |
| Classification： | Adenoviridae, Mastadenovirus, Human adenovirus E |
| Agent： | Human adenovirus 4 |
| Strain： | RI-67 |
| Applications： | Respiratory research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived by adaptation of HEK cell product ATCC® VR-1081™ to HeLa-229 cells (ATCC® CCL-2.1™). ATCC® VR-1081™ and ATCC® VR-1572™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. HEK cells are known to contain Human adenovirus 5 DNA, and Human adenovirus 5 has been detected by PCR in this item. |
| Effect on Host： | CPE, cell rounding and degeneration |
| Recommended Host： | HeLa-229 (ATCC® CCL-2.1™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-7 days |
| Name of Depositor： | WP Rowe, National Institute of Allergy and Infectious Diseases; ATCC® |
| Special Collection： | ATCC |
| Source： | Throat washings of patient, Fort Leonard Wood, Missouri, 1952-1953 |
| References： | Huebner RJ, et al. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N. Engl. J. Med. 251: 1077-1086, 1954. PubMed: 13223958 Werner JH, Hilleman MR. Recovery of new agent from patients with acute respiratory illness. Proc. Soc. Exp. Biol. Med. 85: 183-188, 1954. PubMed: 13134329 |