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| 产品名称： | lambdaSK6 clone 23 |
|---|---|
| 商品货号： | TS135893 |
| Designations： | lambdaSK6 clone 23 |
| Depositors： | ER Zabarovsky, G Winberg, G Klein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 43.2000007629394500 Vector: lambdaSK6 (phage, lambda - replacement) Promoters: Promoter lac Construction: lambda 2001 Marker(s):red+ gam+ Construct size (kb): 43.20000076293945 Features: insert detection: lacZ, Spi+ marker(s): red+ gam+ promoter: lac replicon: lambda |
| Applications： | vector containing primer sites useful for sequencing vector for constructing genomic libraries vector for other uses vector permitting positive selection for inserts vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 13.5, 9.0; EcoRI--20.0, 13.5, 9.0; XhoI--20.0, 13.5, 9.0. Vector permitting positive selection (Spi) of and visual detection (lacZ) of recombinants. Useful for generating jumping and linking libraries. Also permits construction using partial filling-in. The polylinker sites attached to each arm are the same. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7. The order of the major features in this plasmid is: arm - XbaI/MCS/AvrII XbaI - lacZO --> - stuffer - |
| References： | Zabarovsky ER, et al. Lambda SK diphasmids: phage lambda vectors for genomic, jumping, linking and cDNA libraries. Gene 127: 1-14, 1993. PubMed: 8486275 Zabarovsky ER, Winberg GN. Improved methods and new vectors for creating libraries of cloned genes. Sov. Sci. Rev. 11: 1-65, 1992. |
| Shipped： | frozen |