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| 产品名称： | YCF3 |
|---|---|
| 商品货号： | TS135917 |
| Designations： | YCF3 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0999999046325680 Vector: YCF3 (plasmid) Construction: YRp14 Marker(s):URA3,ampR,SUP11 Construct size (kb): 7.099999904632568 Features: marker(s): ampR, URA3, SUP11 replicon: pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; HindIII--7.1; BamHI--7.1; HindIII/BglII--7.1. The sequence to be mapped is cloned into the MCS of both YCF3 (TS135917) and YCF4 (ATCC 77156) (both orientations being produced). NotI is used to linearize the constructs, which are transformed independently into YPH49. YCF3 is used to recover sequences proximal to the mapping sequence. Shuttle chromosome fragmentation vector. Constructed by replacing the 346 bp HindIII/BamHI fragment from YRp14 with a 1.5 kb HindIII/BglII fragment containing the telomere-adjacent sequence (Y) and a polylinker. EcoRI and HindIII sites were removed. The order of the major features in this plasmid is: Y - MCS - SalI - URA3 - SUP11 - rep - pMB1 ori - bla. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Vollrath D, et al. Physical mapping of large DNA by chromosome fragmentation. Proc. Natl. Acad. Sci. USA 85: 6027-6031, 1988. PubMed: 3045811 Gerring SL, et al. Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol. 194: 57-77, 1991. PubMed: 2005810 |
| Shipped： | freeze-dried |