微信小陈
微信小章| 产品名称： | pLOGAN |
|---|---|
| 商品货号： | TS135922 |
| Designations： | pLOGAN |
| Species： | Beet curly top virus |
| Depositors： | DC Stenger |
| Applications： | contains sequence Plant research |
| Vector： | Construct size (kb): 5.73
Construction of the clone:xa0 Plasmid constructed by inserting 3038 bp Sal I linearized viral dsDNA into Sal I site of pUC8vector. |
| Insert： | DNA: cDNA
Insert lengths(kb): 3.04
Tissue: Beet curly top virus |
| Insert Size (kb)： | 3.038 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Plasmid constructed by inserting 3038 bp SalI linearized viral dsDNA into SalI site of pUC8 vector. >95% sequence identity wiith BCTV- California. The clone is infectious. The ATCC sample contains 30ug of purifed plasmid DNA in TE buffer. |
| Classification： | Curtovirus |
| References： | Stenger DC, et al. Genomic characterization of phenotypic variants of beet curly top virus. J. Gen. Virol. 71: 2211-2215, 1990. PubMed: 2230726 Stenger DC. Complete nucleotide sequence of the hypervirulent CFH strain of beet curly top virus. Mol. Plant-Microbe Interact. 7: 154-157, 1994. PubMed: 8167369 Stenger DC, et al. Phylogenetic relationships within the family Potyvirdae: wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology 88: 782-787, 1998. |