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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS135930 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | MKH13 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | DELTA(putPA)101 DELTA(proP)2 DELTA(proU)608 |
| Type Strain： | no |
| Comments： | Phenotype(s) verified: spectinomycin resistance. transformation host deficient in uptake of osmoprotectants, for use with expression vectors pOSEX2 - pOSEX5C (ATCC 87211 - 87216) Derived from E. coli MC4100. |
| Medium： | ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | E Bremer |
| Special Collection： | NCRR Contract |
| References： | Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862 Haardt M, et al. The osmoprotectant proline betaine is a major substrate for the binding-protein-dependent transport system ProU of Escherichia coli K-12. Mol. Gen. Genet. 246: 783-786, 1995. PubMed: 7898450 |