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| 产品名称： | pSP65:pUAS14#4 plasmid in E. coli |
|---|---|
| 商品货号： | TS135954 |
| Designations： | pSP65:pUAS14#4 plasmid in E. coli |
| GenBank Number： | M36646 |
| Species： | Apple scar skin viroid |
| Depositors： | AF Hadidi, United States Department of Agriculture |
| Vector： | Name of construct: pSP65:pUAS14#4 Excise insert: EcoRI Size of construct (kb): 3.3 Markers: ampR Name of vector: pSP65 Intact vector size: 3.010 Type of vector: plasmid Vector end: EcoRI Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI EcoRI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none marker(s): ampR promoter: SP6 replicon: pMB1 terminator: none |
| Insert： | Genome: apple scar skin viroid (ASSV) Genomic copy number: unique Contains complete coding sequence?: U Type of DNA: cDNA Insert end: EcoRI Insert end: EcoRI Insert size (kb): 0.274 Cross references: DNA Seq. Acc.: M36646 |
| Insert Size (kb)： | 0.274 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.3; PstI--3.3; BglI--3.3; HindIII--3.3. Derived from nucleotides 286 to 228 in the sequence of the circular viroid. If the plasmid is linearized with PstI or HindIII, transcription with SP6 polymerase will generate the complement (minus strand). |
| Classification： | Aspcaviroid |
| References： | Hashimoto J, Koganezawa H. Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Res. 15: 7045-7051, 1987. PubMed: 3658673 Ahmed F Hadidi, personal communication |