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微信小章| 产品名称： | cos 7-1 |
|---|---|
| 商品货号： | TS136035 |
| Designations： | cos 7-1 |
| GenBank Number： | U07000 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 48.68000030517578 DESCRIPTION OF VECTOR COMPONENT: Name of vector: svPHEP Intact vector size: 9.201 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI ClaI Polylinker sites: HindIII ClaI EcoRI BamHI Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR replicon: pMB1 Cross references: DNA Seq. Acc.: L19900 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: BCR Other gene symbols: BCR1 Genomic copy number: unique Gene name: breakpoint cluster region Contains complete coding sequence?: N Chromosome: 22; Localization: 22 q11 Tissue: chronic myelogenous leukemia K-562 cell line Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 39.48 Cross references: DNA Seq. Acc.: U07000 Nucleotides 1-39480 of the insert correspond to nucleotides 1-39480 of U07000. Insert lengths(kb): 39.47999954223633 Tissue: chronic myelogenous leukemia K-562 cell line Gene product: breakpoint cluster region BCR Alleles: A1, A1, A2, A2 |
| Insert Size (kb)： | 39.480 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--10.5, 7.0 (triplet), 6.2, 3.5, 2.4, 2.05, 0.98, 0.90, 0.82, 0.68; BglII--8.6, 6.6, 5.8, 5.3, 4.4, 3.3, 3.15, 2.95, 2.3 (doublet), 1.9, 0.54, 0.3; EcoRI--10.0, 7.0 (doublet), 6.2 (doublet), 4.1, 2.9, 2.4, 1.7, 1.1; HindIII--15.0, 11.0, 5.8, 5.6, 4.3, 3.3, 1.8, 1.05, 0.7, 0.48; NotI--uncut; SstI--15.5, 13.0, 10.5, 8.0, 2.3, 0.94, 0.67. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The insert begins 15038 nt upstream of exon 1 and continues past exon 2 into intron 3. The upstream region contains a TATA box, several protein binding sites, a repeat region (13983-14521) and an inverted repeat (16054-16330). |
| References： | Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859 Heisterkamp N, et al. The first BCR gene intron contains breakpoints in Philadelphia chromosome positive leukemia. Nucleic Acids Res. 16: 10069-10081, 1988. PubMed: 3194194 Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185 Bruce A Roe, personal communication |