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微信小章| 产品名称： | 55.E 55E |
|---|---|
| 商品货号： | TS136054 |
| Designations： | 55.E 55E |
| Species： | Homo sapiens, human |
| Depositors： | J Mandel |
| Vector： | Construct size (kb): 4.5 |
| Insert： | DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: DNA Segment, single copy DXS105 Alleles: E1, E2 |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; EcoRI/HindIII--2.7, 1.8; PstI--4.5; XbaI--4.5. When combined with the TaqI RFLP detected by cX55.7, the heterozygosity at DXS105 is about 52%. This probe was derived from cos55 and contains some repetitive sequences. Hybridization should be at 42C in 50% formamide and washes should be at 68C, 0.2X SSC. |
| References： | Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795 Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147 Gejman PV, et al. Manic depressive illness not linked to factor IX region in an independent series of pedigrees. Genomics 8: 648-655, 1990. PubMed: 1980485 Arveiler B, et al. Genetic mapping of the Xq27-q28 region: new RFLP markers useful for diagnostic applications in fragile-X and hemophilia-B families. Am. J. Hum. Genet. 42: 380-389, 1988. PubMed: 2893549 Jean Louis Mandel, personal communication |