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| 产品名称： | pHUMNHE1 |
|---|---|
| 商品货号： | TS136061 |
| Designations： | pHUMNHE1 |
| GenBank Number： | M81768 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 7.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS- Intact vector size: 2.961 Type of vector: phagemid Vector end: HindIII Vector end: SmaI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, ->, 3-459 primer site: M13-20, -> promoter for in vitro transcription: T7, -> insert detection: lacZ, MCS: SacI...KpnI, ->, 657-759 promoter for in vitro transcription: T3, primer site: reverse, replicon: pMB1, 1032-1972 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SLC9A1 Other gene symbols: APNH; NHE1 Gene name: solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 p36.1-p35 Tissue: kidney cortex Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: Modification: blunt ended Insert size (kb): 4.0 Cross references: DNA Seq. Acc.: M81768 Insert lengths(kb): 4.0 Tissue: kidney cortex Gene product: NA(+)/H(+) antiporter 1( Na+/H+ antiporter, Na+/H+ antiporter protein) APNH |
| Insert Size (kb)： | 4.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.2, 3.1; BamHI--3.4, 2.0, 1.5, 0.41; PstI--7.2; SacI--7.2; PvuII--2.7, 2.45, 1.10, 0.56, 0.28. Constructed from a 520 bp HindIII/PstI PCR product and the large PstI/EcoRI fragment from HK-3.5. The SmaI site was lost during cloning, and the vector SacI site was deleted. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.38, 2.3, 3.7; PstI--0.52; SacI--1.1; PvuII--2.1, 2.6, 2.9, 3.9. |
| References： | Takaichi K, et al. Cloning, sequencing, and expression of Na+-H+ antiporter cDNAs from human tissues. Am. J. Physiol. 262: C1069-C1076, 1992. PubMed: 1314485 David G Warnock, personal communication |