Construct size (kb): 3.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Tissue: BL29 cell line
Disease: Burkitts lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.5
Cross references: DNA Seq. Acc.: T25850; EST: EST220; Primer: T7
DNA Seq. Acc.: T25851; EST: EST220R; Primer: T3

Insert lengths(kb): 0.5

Tissue: BL29 cell line Burkitts lymphoma

Disease: Burkitts lymphoma

Gene product: DNA Segment, single copy, expressed

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--3.5; EcoRI--3.0, 0.5; HindIII--3.5; PstI--3.5; SacI--3.5.