产品名称: | pbs/PSP94 |
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商品货号: | TS136164 |
Designations: | pbs/PSP94 |
Species: | Homo sapiens, human |
Vector: | Construct size (kb): 3.799999952316284 |
Insert: | DNA: cDNA Insert lengths(kb): 0.5400000214576721 Tissue: prostate Gene product: microseminoprotein, beta-(prostatic secretory protein) MSMB |
Insert Size (kb): | 0.540 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
Comments: | Restriction digests of the clone give the following sizes (kb): PstI/SstI--3.25, 0.54; PstI--3.8; SstI--3.7; XhoI--uncut; KpnI/BamHI--3.2, 0.5; RsaI--2.25, 0.72 (doublet); XhoI--1.5, 1.0, 0.8. The insert contains EcoRII (4), MboI (2), NsiI (2), HinfI (2), XhoII (1), RsaI (1), TaqI (1), AvaII (1), and HaeIII (2) sites. The insert consists of 483 bp of cDNA, 53 bp of dG/dC, and 17 bp of poly(A). The insert encodes the complete prepolypeptide of 114 amino acids and includes 11 bp 5 noncoding and about 128 bp 3 noncoding sequence. There is no AATAAA polyadenylation signal although there is a AGTAAA. Detects human genomic restriction fragments of the following sizes (kb): EcoRI--3.4 (and a weaker band of 3.0 kb that may be artifactual); BamHI--3.1, 0.5; PvuII--2.8, 0.8, 0.75. |
References: | Mbikay M, et al. Molecular cloning and sequence of the cDNA for a 94-amino-acid seminal plasma protein secreted by the human prostate. DNA 6: 23-29, 1987. PubMed: 3829888 Nolet S, et al. Rapid evolution of prostatic protein PSP94 suggested by sequence divergence between Rhesus monkey and human cDNAs. Genomics 9: 775-777, 1991. PubMed: 2037304 Majambu Mbikay, personal communication |