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| 产品名称： | Polytomella caeca Pringsheim |
|---|---|
| 商品货号： | TS136231 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Type Strain： | no |
| Comments： | Preservation Life cycle |
| Medium： | ATCC® Medium 351: Hutners medium for Euglena |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C. |
| Subcultivation： | Protocol: ATCCNO: 12910 SPEC: Aseptically add 0.5 ml of the growth medium to the inner shell vial containing the filter paper and incubate at 20C in the dark. Maintain the level of the medium in the vials at 0.5 ml during the incubation period. Observe daily for motile cells (motile cells should be observed within 6-8 days after rehydration). When numerous cells are evident, transfer the entire liquid contents of the vial (not the filter paper) to a tube of fresh medium and incubate at 25C. |
| Name of Depositor： | DL Wise |
| Chain of Custody： | ATCC < |
| References： | Reed RB, et al. Preservation of encysted Polytomella. J. Gen. Microbiol. 97: 29-34, 1976. PubMed: 186558 Lewis E, et al. Life cycle of Polytomella caeca (phytomonadida, Polyblepharidae). J. Protozool. 21: 647-649, 1974. |