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微信小章| 产品名称： | vW-8 pvW |
|---|---|
| 商品货号： | TS136274 |
| Designations： | vW-8 pvW |
| Species： | Homo sapiens, human |
| Applications： | There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5 end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful. For unique sequence probes from the 3 end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful. |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 8.800000190734863 Tissue: endothelium Gene product: von Willebrand factor pseudogene VWF Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2, , , , A1, A2, A1, A1, A2, A2 |
| Insert Size (kb)： | 8.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--9.4, 2.9; BamHI--5.8, 5.8; HindIII--6.6, 5.3; PstI--3.4, 1.8, 1.1, 0.8, + others; purified DNA: HindIII--6.64, 5.7; SacI--6.6, 3.8, 2.0; BamHI--6.2 doublet; EcoRI--14; EcoRI/SacI--3.8, 3.4, 3.0. 1.0. The insert contains the following singly cutting sites: BamHI, EcoRV, HgaI, HindIII, MspI, NaeI, PvuI, RshI, Tth111II, XbaI, XmaIII, XmnI, XorII. The following enzymes do not cut vW-8: AhaIII, AsuII, BclI, BssHII, HpaI, MluI, NdeI, NotI, NruI, SalI, SfiI, SnaBI. This is essentially a full-length cDNA clone except for about 150 bp 5 untranslated and 100 bp 3 untranslated sequence. There seems to be a partial pseudogene for VWF detected by sequences from about 2.5-4.5 kb of the cDNA. For unique sequence probes from the 5 end, EcoRI/HindIII or EcoRI/BglII or EcoRI/XmaIII fragments are useful. For unique sequence probes from the 3 end, the fragment from NarI or XmnI to SalI (in the polylinker) is useful. Sequences at the 3 end show homology to vole, rat, hamster, monkey, and mouse but not quail. Codominant segregation for the TaqI polymorphism has been demonstrated in 4 families. The BamHI polymorphism is detected with a 1.9 kb SacI fragment of the cDNA. The EcoRI polymorphism is detected by a 3.6 kb XmnI/SalI fragment of this clone. Enzyme(s) not detecting polymorphism (for the 1.9 kb SacI fragment probe): AvaI, BglII, BstEII, DraI, EcoRI, HindIII, KpnI, PstI (tested in 13-15 unrelated Caucasians). Enzyme(s) not detecting polymorphism: BglII, EcoRI, SacI, TaqI. |
| References： | Marchetti G, et al. Two additional TaqI RFLPs in von Willebrand factor gene (VWF) and pseudogene. Nucleic Acids Res. 17: 3329, 1989. PubMed: 2566970 Ewerhardt B, et al. An EcoRI polymorphism in the human von Willebrand factor (vWF) gene. Nucleic Acids Res. 17: 5416, 1989. PubMed: 2569713 Driscoll CM, et al. A KpnI DNA polymorphism in the human von Willebrand factor (VWF) gene. Nucleic Acids Res. 18: 4968, 1990. PubMed: 1975687 Bernardi F, et al. Two TaqI RFLPs in the human von Willebrand factor gene. Nucleic Acids Res. 15: 1347, 1987. PubMed: 2881274 Nishino K, Lynch DC. A polymorphism of the human von Willebrand factor (vWF) gene with BamHI. Nucleic Acids Res. 14: 4697, 1986. PubMed: 3012477 Collins CJ, et al. Molecular cloning of the human gene for von Willebrand factor and identification of the transcription initiation site. Proc. Natl. Acad. Sci. USA 84: 4393-4397, 1987. PubMed: 3496594 Bonthron D, et al. Nucleotide sequence of pre-pro-von Willebrand factor cDNA. Nucleic Acids Res. 14: 7125-7127, 1986. PubMed: 3489923 |