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| 产品名称： | 12f p12f |
|---|---|
| 商品货号： | TS136276 |
| Designations： | 12f p12f |
| Species： | Homo sapiens, human |
| Depositors： | J Weissenbach |
| Vector： | Construct size (kb): 9.359999656677246 |
| Insert： | DNA: genomic Insert lengths(kb): 5.0 Gene product: DNA Segment, single copy DYS11 Alleles: B2, B1, A1, A2 |
| Insert Size (kb)： | 5.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | BglII digestion of recombinant plasmid gives fragments of 5.2, 2.2, and 1.9 kb. Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 4.4; BamHI--9.8; PstI--6.2, 3.7; PvuI--9.8; BamHI/PstI--3.7, 3.4, 2.5. The depositor indicates that the BglII fragment sizes are slightly overestimated in this reference. EcoRI detects numerous bands, 3 of which are Y-specific. Male-specific band A (5 kb) is the cognate one and may correspond to 2 to 3 copies; maps to Yq11 and Yq12. Band B appears as 4.5 or 3 kb; maps to Yq11. Band C is about 1 kb and maps to Yq11. TaqI detects a 10 kb band equivalent to EcoRI band B (4.5 kb allele), an 8.5 kb band equivalent to EcoRI band A and B (3.0 kb allele), and a 3.8 kb band equivalent to EcoRI fragment A. The BglII subfragment of 2.2 kb preferentially detects the Y-specific bands. Use stringent wash conditions (0.1X SSC, 68C). |
| References： | Vergnaud G, et al. A deletion map of the human Y chromosome based on DNA hybridization. Am. J. Hum. Genet. 38: 109-124, 1986. PubMed: 3004206 Casanova M, et al. A human Y-linked DNA polymorphism and its potential for estimating genetic and evolutionary distance. Science 230: 1403-1406, 1985. PubMed: 2999986 Jean Weissenbach, personal communication |