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| 产品名称： | pJM128 plasmid in E. coli |
|---|---|
| 商品货号： | TS136407 |
| Designations： | pJM128 plasmid in E. coli |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | TA Donlon |
| Vector： | Total size of construct (kb): 6.95 Marker: ampR Vector: pBR322 Vector type: plasmid Vector size (kb): 4.4 Markers: ampR |
| Insert： | Insert size (kb): 2.55 Gene symbol: D8Z2 HGML probe ID: p03155 DNA: genomic DNA Gene product: Alphoid repeat Source: Human chromosome 8 cen |
| Insert Size (kb)： | 2.550 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Frozen glycerol stock of E. coli containing the plasmid |
| Comments： | The insert contains RsaI (5), PstI (1), and XbaI (2) sites. Restriction digests of the clone give the following sizes (kb): HindIII--4.3, 2.5; PstI--4.0, 2.9; XbaI--6.3, 0.66; XbaI/HindIII--4.3, 1.4, 0.66; XbaI/PstI--4.0, 2.2, 0.66. This is a subclone of lambda285-1225 (the central 2.55 kb HindIII fragment) and contains 15 repeat monomers. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Donlon T, et al. Alpha satellite-like sequences at the centromere of chromosome #8. Am. J. Hum. Genet. 39: A196, 1986. Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938 Timothy A Donlon, personal communication |