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| 产品名称： | phIL1AcDNA human IL-1alpha cDNA |
|---|---|
| 商品货号： | TS136435 |
| Designations： | phIL1AcDNA human IL-1alpha cDNA |
| GenBank Number： | M28983 |
| Species： | Homo sapiens, human |
| Depositors： | U Gubler |
| Vector： | Construct size (kb): 6.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: peripheral blood cells (LPS activated) Gene product: interleukin 1, alpha IL1 |
| Insert Size (kb)： | 2.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following restriction sites (nt positions according to the published sequence): EcoRI--434; HindIII--227; ScaI--1670. Restriction digests of the clone give the following sizes (kb): BamHI--4.0, 2.4; EcoRI--4.2, 2.2; HindIII--4.1, 2.3; SacI-- uncut; PstI--6.4. Contains the complete coding sequence (nt 38-852). pMG-5 is a pBR322 derivative in which the sequence adjacent to the EcoRV site has been modified (GGATATCG to GGATATCC) to allow generation of flanking BamHI sites after homopolymeric tailing EcoRV-cut vector with Cs. |
| References： | Gubler U, et al. Recombinant human interleukin 1 alpha: purification and biological characterization. J. Immunol. 136: 2492-2497, 1986. PubMed: 3485152 Ueli Gubler, personal communication |