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| 产品名称： | pCM189 |
|---|---|
| 商品货号： | TS136438 |
| Designations： | pCM189 |
| Depositors： | E Herrero |
| Biosafety Level： | 1 |
| Host： | E. coli DH5α |
| Vector Information： | Size (kb): 8.37
Vector: pCM189 (plasmid)
Promoters: Promoter CMV
Marker(s): URA3,ampR
Construct size (kb): 8.37
Features: marker(s): URA3 marker(s): ampR operator: tetO - seven repeats other: tTA transcriptional activator promoter: CMV promoter: CYC1 replicon: ARS1 replicon: pMB1 MCS: BamHI...PstI terminator: ADH1 terminator: CYC1 centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--8.4; PstI--8.4; BamHI/XhoI--7.7, 0.67. Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672 |
| Related Products： | Component of: ATCC 87663 |
| Shipped： | frozen |