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微信小章| 产品名称： | Hf677 pHF677 |
|---|---|
| 商品货号： | TS136499 |
| Designations： | Hf677 pHF677 |
| GenBank Number： | K01228 |
| Species： | Homo sapiens, human |
| Depositors： | M Chu |
| Vector： | Construct size (kb): 6.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.799999952316284 Tissue: human fibroblast Gene product: collagen, type I, alpha 1 COL1A1 |
| Insert Size (kb)： | 1.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.5, 0.3; HindIII--6.2; PstI--6.2; BamHI--6.2; PstI/BamHI--3.0, 2.7. The insert contains the following restriction sites (number): AvaI (2), HinfI (3), TaqI (6), XhoI (1). The insert encodes amino acids 787 to the carboxy terminus, and includes 270 nucleotides 3 untranslated. The insert detects the genomic HindIII fragments of the following sizes (kb): human--6.0; Chinese hamster--8.8, 2.8; mouse--2.7, 2.0. The probe detects genomic EcoRI fragments of 19.0 and 5.4 kb in the human, 11.5 kb in the rat, and 14.0 kb in the mouse. The probe detects mRNA of 5.8 and 4.8 kb in human fibroblasts. |
| References： | Chu ML, et al. Cloning and characterization of five overlapping cDNAs specific for the human pro alpha1(I) collagen chain. Nucleic Acids Res. 10: 5925-5934, 1982. PubMed: 6183642 Huerre C, et al. Human type I procollagen genes are located on different chromosomes. Proc. Natl. Acad. Sci. USA 79: 6627-6630, 1982. PubMed: 6292910 Leach RJ, et al. Physical mapping of human chromosome 17 using fragment-containing microcell hybrids. Genomics 5: 167-176, 1989. PubMed: 2571568 Bernard MP, et al. Nucleotide sequences of complementary deoxyribonucleic acids for the proalpha1 chain of human type I procollagen. Statistical evaluation of structures that are conserved during evolution. Biochemistry 22: 5213-5223, 1983. PubMed: 6689127 Mon-Li Chu, personal communication |