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| 产品名称： | pGH6 |
|---|---|
| 商品货号： | TS136527 |
| Designations： | pGH6 |
| Depositors： | Genentech, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli 294; GMBH K-12 294 Distribution host: Escherichia coli 294; GMBH K-12 294 |
| Vector Information： | Size (kb): 4.5999999046325680 Vector: pGH6 (plasmid) Promoters: Promoter lacUV5 Construction: pBR322, pKB268 Marker(s):ampR,tetR Construct size (kb): 4.599999904632568 Features: marker(s): ampR, tetR promoter: lacUV5 replicon: pMB1 |
| Applications： | expression vector |
| Comments： | pGH6 is available in a bacteria culture as TS136527 or as purified plasmid DNA as ATCC 40012. Expression vector. The EcoRI site is downstream of the lacUV5 promoters. This was constructed by inserting a 285 bp EcoRI fragment from pKB268 (containing 2 95 bp lac UV5 promoters separated by heterologous DNA) into the EcoRI site of pBR322. The EcoRI site distal to the tetR gene was destroyed. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Goeddel DV, Heyneker HL. Method of constructing a replicable cloning vehicle having quasi-synthetic genes. US Patent 4,342,832 dated Aug 3 1982 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |