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微信小章| 产品名称： | pH3H2 |
|---|---|
| 商品货号： | TS136563 |
| Designations： | pH3H2 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.0 Gene product: macrophage stimulating 1 (hepatocyte growth factor-like)(hepatocyte growth factor-like) D1S1 Alleles: B2, B2, B2, B2, B1, B1, B1, B1, A2, A2, A1, A2, A1, A1 |
| Insert Size (kb)： | 2.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Insert contains AvaI (2), PstI, KpnI (2), and BamHI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; PstI--3.0, 2.6; BamHI--3.9, 1.7; HindIII--3.5, 1.95; AvaI--2.7, 1.5, 1.2. Detects loss of heterozygosity in renal cell carcinoma. Derived from Ch4A-lambdaH3 (DNF15S2, formerly D1S1, p01970). This probe detects a genomic HindIII fragment of 2 kb from chromosome 3 and genomic HindIII fragments of 3.8 and 8 kb from chromosome 1. The copy number of the fragments on chromosome 1 is 6-8 per haploid genome. This probe detects a StuI RFLP mapping to 1p36 and a HindIII RFLP mapping to 3p21. Can detect loss of heterozygosity in lung cancers. The StuI polymorphism shows autosomal dominant inheritance because of the repetitive nature of the locus. Band sizes are 3.8 kb, 3.6 kb, 2.0 kb, and 1.2 + 0.6 kb. |
| References： | Leduc F, et al. Loss of heterozygosity in a gene coding for a thyroid hormone receptor in lung cancers. Am. J. Hum. Genet. 44: 282-287, 1989. PubMed: 2536219 Welch HM, et al. Transposition, amplification, and divergence in the origin of the DNF15 loci, a polymorphic repetitive sequence family on chromosome 1 and 3. Genomics 5: 423-430, 1989. PubMed: 2575580 Carritt B, et al. The D1S1 locus is part of a small dispersed sequence family (DNF15). Cytogenet. Cell Genet. 40: 559-600, 1985. Zbar B, et al. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. Nature 327: 721-724, 1987. PubMed: 2885753 Kok K, et al. Deletion of a DNA sequence at the chromosomal region 3p21 in all major types of lung cancer. Nature 330: 578-581, 1987. PubMed: 2825033 Benjamin Carritt, personal communication |