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| 产品名称： | pUMA13 |
|---|---|
| 商品货号： | TS136579 |
| Designations： | pUMA13 |
| Depositors： | UA Heinlein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0000000000000000 Vector: pUMA13 (plasmid) Promoters: Promoter for in vitro transcription SP6 Construction: pSP64 Marker(s):ampR Construct size (kb): 3.0 Features: initiation codon: ATG marker(s): ampR promoter for in vitro transcription: SP6 replicon: pMB1 MCS: EcoRI...NotI restriction site: NcoI signal peptide coding region: NCAM stop codons: 3 |
| Applications： | construct to generate mRNA that can be expressed in Xenopus oocytes vector for constructing cDNA libraries vector permitting RNA synthesis in vitro vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; NotI--3.0; XhoI--3.0. Vector designed for in vitro transcription of cloned inserts encoding open reading frames. Transcribed RNA encodes the murine NCAM signal sequence for protein export and will contain the authentic 5-UTR of X. laevis beta globin on the 5 end. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614 |
| Shipped： | freeze-dried |