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| 产品名称： | pAU21 |
|---|---|
| 商品货号： | TS136679 |
| Designations： | pAU21 |
| Depositors： | H Okayama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.3000001907348630 Vector: pAU21 (plasmid) Construction: pFL20 Marker(s):URA3,tetR Construct size (kb): 5.300000190734863 Features: marker(s): tetR, URA3 replicon: pMB1, ars |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--2.6 (doublet); PstI--5.2. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Contains the ars sequence from Schizosaccharomyces pombe and the URA3 gene from Saccharomyces cerevisiae. Insertions at the BamHI or SalI sites inactivate tetracycline resistance. Shares pBR322 sequences from the PvuII site to the ScaI site with pcD. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111 |
| Shipped： | freeze-dried |