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| 产品名称： | pTC191 |
|---|---|
| 商品货号： | TS136729 |
| Designations： | pTC191 |
| Depositors： | A Talavera |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7000000476837160 Vector: pTC191 (plasmid) Construction: pUC19 Marker(s):ampR Construct size (kb): 2.700000047683716 Features: insert detection: lacZ marker(s): ampR replicon: pMB1 MCS: BamHI...HindIII |
| Applications： | vector containing primer sites useful for sequencing vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.15; NcoI--2.7; PvuII--2.3, 0.40. The plasmid contains the following restriction sites (approximate kb from nt 1): BglI--0.24, 1.85; NcoI--0.45; PvuI--0.28, 2.10; PvuII--0.31, 0.67. Expression vector designed for converting restriction sites at the end of inserts. Also permits insert detection by lacZalpha complementation and sequencing from M13 universal and reverse primers. Constructed from pUC19 by modification of the multiple cloning site to include some unique cloning sites surrounded by some repeated sites. Pairs of identical restriction sites are in the same reading frame to maintain lacZ insert detection regardless of the enzyme used. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | DeLaFuente A, et al. Restriction end-converting vectors with tandem repeated multiple cloning sites. Gene 139: 83-86, 1994. PubMed: 8112593 |
| Shipped： | freeze-dried |