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| 产品名称： | pBKV (35-1) |
|---|---|
| 商品货号： | TS136737 |
| Designations： | pBKV (35-1) |
| GenBank Number： | J02039 |
| Species： | BK polyomavirus, BK Virus |
| Depositors： | PM Howley |
| Vector： | Construct size (kb): 9.30 Name of construct: pBKV (35-1) Excise insert: BamHI Size of construct (kb): 9.3 Markers: ampR Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | Genome: BK virus Contains complete coding sequence?: U Strain: MM Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 5.0 Cross references: DNA Seq. Acc.: J02039 |
| Insert Size (kb)： | 4.963 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | SPECIAL CHARACTERISTICS: ORIGINAL SOURCE: Lysate of infected tissue culture cells. Entire genomic DNA linearized at BamHI site into BamHI site of pBR322 SPECIAL CHARACTERISTICS: The entire viral genome was isolated from an infected human brain. BK virus was linearized with BamHI before cloning. |
| Classification： | Polyomaviridae, Polyomavirus |
| References： | Yang RC, Wu R. BK virus DNA: complete nucleotide sequence of a human tumor virus. Science 206: 456-462, 1979. PubMed: 228391 |