产品名称: | pEco63 plasmid in E. coli |
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商品货号: | TS136740 |
Designations: | pEco63 plasmid in E. coli |
Depositors: | University of California |
Applications: | Blood borne research Sexually Transmitted Disease Research |
Vector: | Total sizexa0of construct (kb): 9.2 Marker: ampR Vector:xa0 pBR325 Vector type: plasmid Vector size (kb): 5.996 Markers: ampR, tetR Vector ends: EcoRI Replicon: pMB1 |
Insert: | Insert size (kb): 3.2 Gene: Hepatitis B virus DNA: Genomic Insert ends: EcoRI |
Insert Size (kb): | 3.200 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Glycerol stock of E. coli containing the plasmid |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.5; HindIII--10. Full-length clone (insert size 3.2 kb). This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims. Constructed by inserting a full-length genome of hepatitis B cut with EcoRI into EcoRI site of pBR325 Constructed by inserting a full-length genome of hepatitis B virus cut with EcoRI into the EcoRI site of pBR325. |
References: | Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336 Kate H Murashige, personal communication |
Disclosure: | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |