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微信小章| 产品名称： | Trypanosoma chattoni Mathis and Leger |
|---|---|
| 商品货号： | TS136900 |
| Strain Designations： | 314, clone AC |
| Application： | Karyotype analysis of anuran trypanosomes |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Rana pipiens, Anaoka County, MN, 1950 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Phylogenetic relationships among anuran trypanosomes Species compared using RAPDs Karyotype analysis of anuran trypanosomes |
| Medium： | ATCC® Medium 1011: Diphasic blood agar medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor： | LS Diamond |
| Year of Origin： | 1950 |
| References： | Clark CG, et al. Phylogenetic relationship among anuran Trypanosoma as revealed by riboprinting. J. Eukaryot. Microbiol. 42: 92-96, 1995. PubMed: 7728144 Lun ZR, Desser SS. Analysis of isolates within species of anuran trypanosomes using random amplified polymorphic DNA. Parasitol. Res. 82: 22-27, 1996. PubMed: 8825440 Lun ZR, Desser SS. Karyotype analysis of anuran trypanosomes by pulsed-field gradient gel electrophoresis. J. Parasitol. 81: 1018-1020, 1995. PubMed: 8544044 |