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| 产品名称： | Leishmania donovani (Laveran and Mesnil) Ross |
|---|---|
| 商品货号： | TS137002 |
| Strain Designations： | 3K |
| Application： | Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | human, Kenya, 1962 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Amastigotes. |
| Growth Conditions： | Duration: in vivo, golden hamster Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum. |
| Subcultivation： | Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum. |
| Name of Depositor： | R Herman |
| Chain of Custody： | ATCC < |
| Year of Origin： | 1962 |
| References： | Stauber LA. Characterization of strains of Leishmania donovani. Exp. Parasitol. 18: 1-11, 1966. PubMed: 5909670 |