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| 产品名称： | pLKT5 |
|---|---|
| 商品货号： | TS137035 |
| Designations： | pLKT5 |
| GenBank Number： | M20730 |
| Species： | Mannheimia haemolytica (Newsom and Cross) Angen et al. |
| Depositors： | RY Lo |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: cDNA Insert lengths(kb): 4.400000095367432 Gene product: leukotoxin lktA |
| Insert Size (kb)： | 4.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert includes the following sites, numbered beginning at -469 for the EcoRV site: PvuI--158, HincII--227, BglII--346, PvuII--1252, PstI--1650, EcoRV--2683, XbaI--3920. Restriction digests of the clone give the following sizes (kb): SalI--9.4; EcoRV/SalI--4.4, 3.4, 1.3; EcoRV--5.8, 3.2. The insert includes 2 open reading frames; one for lktC encoding a protein of 19.8 kDa and one for lktA encoding a protein of 101.9 kDa. HB101 transformed with the plasmid shows the 100 kDa protein on western blots, but not the 20 kDa protein. |
| References： | Lo RY, et al. Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1. Infect. Immun. 55: 1987-1996, 1987. PubMed: 3040588 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |