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微信小章| 产品名称： | pAT-A K02403, SGC31623 |
|---|---|
| 商品货号： | TS137128 |
| Designations： | pAT-A K02403, SGC31623 |
| GenBank Number： | K02403 |
| Species： | Homo sapiens, human |
| Depositors： | MC Carroll |
| Applications： | The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI. |
| Vector： | Construct size (kb): 8.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 5.400000095367432 Tissue: liver Gene product: complement component 4A C4A Alleles: D1, D2, A2, A1 |
| Insert Size (kb)： | 5.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.5 (doublet), 2.3; HindIII--9.5; HindIII/EcoRI--6.4, 2.9; HindIII/XhoI--4.1, 3.0, 2.2; PvuII--5.2, 2.3, 1.1, 0.5, 0.4; HindIII/SalI--6.3, 3.3. Contains the entire coding sequence of pro C4A including approximately 60 bp 5 untranslated and approximately 140 bp 3 untranslated sequence plus poly(A). The insert contains an internal BamHI site. The sequence is nearly identical to the C4B gene product. The BglII RFLP is detected using the 400 bp BamHI/KpnI 5 fragment of pAT-A. The polymorphic site maps 2.5 kb upstream from C4A. The 4.2 kb allele segregates with F allele of factor B and the 6.6 kb TaqI fragment in C2. The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI. Enzyme(s) not detecting polymorphism: TaqI, BamHI, KpnI, HindIII, SalI, ClaI. |
| References： | Schneider PM, et al. Polymorphism of the human complement C4 and steriod 21-hydroxylase genes. J. Clin. Invest. 78: 650-657, 1986. PubMed: 3018042 Belt KT, et al. The structural basis of the multiple forms of human complement component C4. Cell 36: 907-914, 1984. PubMed: 6546707 Palsdottir A, et al. BglII polymorphic site upstream to the human complement component C4A gene. Nucleic Acids Res. 15: 2395, 1987. PubMed: 2882470 McLean RH, et al. Restriction fragment analysis of duplication of the fourth component of complement (C4A). Genomics 2: 76-85, 1988. PubMed: 2838414 |