产品名称: | pE25.b pBS700 |
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商品货号: | TS137150 |
Designations: | pE25.b pBS700 |
Species: | Homo sapiens, human |
Depositors: | IE Scheffler |
Vector: | Construct size (kb): 3.4760000705719 |
Insert: | DNA: genomic Insert lengths(kb): 0.699999988079071 Tissue: DNA from hamster/human XJM12.1.3 hybrid cell line Gene product: DNA Segment, repetitive D1Z7 |
Insert Size (kb): | 0.700 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.7; BamHI--3.2, 0.4; HindIII--3.6; KpnI--3.6; HincII--3.1, 0.36. This insert is a dimer of the repeat of pE25.a (ATCC 61304, 61305). It lacks the EcoRI site in the middle and has several other base changes. |
References: | Carine K, et al. Molecular characterization of human minichromosomes with centromere from chromosome 1 in human-hamster hybrid cells. Somatic Cell Mol. Genet. 15: 445-460, 1989. PubMed: 2781415 |