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| 产品名称： | pAMY8 |
|---|---|
| 商品货号： | TS137191 |
| Designations： | pAMY8 |
| GenBank Number： | X03236 |
| Species： | Bacillus licheniformis (Weigmann) Chester |
| Depositors： | T Thiel |
| Applications： | contains sequence amylase, alpha 1A; salivary Amylase, salivary, alpha-1A |
| Vector： | Construct size (kb): 6.300000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 3.299999952316284 Tissue: Bacillus licheniformis Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A) amyE Target Gene: amylase, alpha 1A; salivary |
| Insert Size (kb)： | 3.300 |
| Media： | ATCC Medium 1065 (see below) plus kanamycin (50 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): ClaI--4.4, 2.0; EcoRI--6.5; HindIII--3.2 (doublet). Alpha amylase expression expressed in Escherichia coli, NPT encodes kanamycin resistance. Insert includes more than one sequenced gene. |
| References： | Elhai J, Wolk CP. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138, 1988. PubMed: 2851487 |