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| 产品名称： | pMMB66EH |
|---|---|
| 商品货号： | TS137216 |
| Designations： | pMMB66EH |
| Depositors： | E Lanka |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 |
| Vector Information： | Size (kb): 8.81
Vector: pMMB66EH (plasmid)
Promoters: Promoter tac
Construction: RSF1010, pKK223-3, M13mp8 polylinker, lacIq
Marker(s): ampR
Promoter: tac
Replicon: RSF1010, oriV, oriT Repressor gene: lacIq Transcription terminator: rrnB |
| Applications： | expression vector shuttle vector |
| Comments： | Contains the following sites separated by (bp): EcoRI-764 -AhaIII-97 -ScaI-112 -PvuI-2101 -AccI-1848 -ScaI-55 -AccI-2039 -BstEII-372 -PvuII-93 -PvuII-94 -HpaI-320 -BstEII-182 -MluI-736 -EcoRI. Restriction digests of the clone give the following sizes (kb): AccI--3.9, 3.0, 1.9; BamHI--8.8; HindIII/PvuII--7.6, 1.3. In the pMMB66 (TS137216, 37621) and pMMB67 (ATCC 37622, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter. An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration. Can be mobilized by conjugative IncP helper plasmids e.g. pRK2013 (ATCC 37159), pUB307 into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria. The sequence of RSF1010 is given in this reference. A 1246 bp lacIQ HindIII fragment was inserted at XmaIII/BamHI of pKK223-3. The 2995 bp NruI/AhaIII fragment of the resulting plasmid was cloned between PstI (bp 7768) and PvuII (bp 1951) of RSF1010. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457 Scholz P, et al. Complete nucleotide sequence and gene organization of the broad-host-range plasmid RSF1010. Gene 75: 271-288, 1989. PubMed: 2653965 Erich Lanka, personal communication |
| Shipped： | frozen |
| Shipping Information： | Frozen glycerol stock of E. coli containing the plasmid |