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| 产品名称： | Hybri-Care Medium |
|---|---|
| 商品货号： | TS137228 |
| Formulation： | Formulation |
| Product Format： | 1 Package (prepares 1L of medium) |
| Storage Conditions： | Store medium at 2°C to 8°C in the dark when not in use. |
| Applications： | Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning. |
| Comments： | Hybri-Care medium is based on the formulation used by D.H. Sachs and collaborators for propagation of hybridomas and other fastidious cell lines. Ingredients are essentially those of a modified DMEM and NCTC 135 plus insulin. A combined buffering system of HEPES and added NaHCO3 enables the medium to be used at all stages of hybridoma production from fusion to cloning, including single-cell subcloning. When used with 10% fetal bovine serum, Hybri-Care repeatedly has been found to support vigorous growth of most hybridomas in the ATCC collection. Ozato, K., et al. J. Immunology 124: 533-540, 1980. |
| Biosafety Level： | N/A |