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| 产品名称： | pBS-hTOP2 UTR-03525, WI-7251 |
|---|---|
| 商品货号： | TS137238 |
| Designations： | pBS-hTOP2 UTR-03525, WI-7251 |
| GenBank Number： | J04088 |
| Species： | Homo sapiens, human |
| Depositors： | JC Wang |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: cDNA Insert lengths(kb): 5.599999904632568 Gene product: DNA topoisomerase II, alpha, 170kDa( DNA Topoisomerase II alpha, DNA topoisomerase II, 170 kD, topoisomerase (DNA) II alpha (170kD)) TOP2 |
| Insert Size (kb)： | 5.600 |
| Media： | ATCC® Medium 1945: Terrific broth (ATCC medium 1743) with 200 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: Frozen |
| Comments： | Restriction digests of the clone give the following sizes (kb): MluI/XhoI--5.6, 3.0; EcoRI--4.3, 3.2, 1.1; ClaI--8.6; HindIII--4.4, 4.2. The insert contains the following restriction sites (approximate kb from the 5 MluI site): BamHI--0.74, 3.25, 4.27; EcoRI--1.3, 4.3; HindIII--1.5; BglII--2.14, 5.2. Contains the complete cDNA. Constructed from lambdahTOP2-37, lambdahTOP2-11, and lambdahTOP2-Z2, joined at the BglII sites. An MluI linker was added to the filled-in NcoI site at the 5 end, and a MluI linker was inserted into the SmaI site of the vector. The original clones were derived from a HeLa cell library (the 2 5 clones) and a HepG2 library (the 3 clone). |
| References： | Tsai-Pflugfelder M, et al. Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22. Proc. Natl. Acad. Sci. USA 85: 7177-7181, 1988. PubMed: 2845399 James C Wang, personal communication |