产品名称: | pEFD33.2 RW-121 |
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商品货号: | TS137248 |
Designations: | pEFD33.2 RW-121 |
Species: | Homo sapiens, human |
Applications: | RFLPs observed under normal stringency (0.1X SSC). |
Vector: | Construct size (kb): 5.900000095367432 |
Insert: | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: DNA Segment, single copy D12S14 Alleles: C2, B1, B2, C1, A4, A2, A3, A1 |
Insert Size (kb): | 3.200 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | Restriction digests of the clone give the following sizes (kb): PstI/EcoRI--2.7, 1.3, 1.3, 0.5; HindIII/EcoRI--2.7, 2.5, 0.5; BamHI/HindIII--2.7, 2.0, 1.2; SalI--uncut. RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: RsaI, BglII, PstI, PvuII, BamHI, HindIII. |
References: | Labuda M, et al. Mapping autosomal recessive vitamin D deficiency type I to chromosome 12q14 by linkage analysis. Am. J. Hum. Genet. 47: 28-36, 1990. PubMed: 1971995 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pEFD33.2 on chromosome 12 D12S14. Nucleic Acids Res. 16: 778, 1988. PubMed: 2893346 Peter J OConnell, personal communication |