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| 产品名称： | c9871 |
|---|---|
| 商品货号： | TS137286 |
| Designations： | c9871 |
| GenBank Number： | U18530 U18779 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | R Hyman |
| Vector： | Construct size (kb): 44.43000030517578 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pHC79 Intact vector size: 6.430 Type of vector: cosmid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI Polylinker sites: Construction: pBR322, Charon 4A Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, tetR replicon: pMB1 terminator: none Cross references: DNA Seq. Acc.: L08873 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: CUP5 Gene name: vacuolar ATP synthase, 16 kDa proteolipid subunit; dicyclohexylcarbodiimide binding subunit Contains complete coding sequence?: Y Chromosome: V Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: RIP1 Gene name: Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex Contains complete coding sequence?: Y Chromosome: V Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: URA3 Gene name: orotidine-5-phosphate decarboxylase Contains complete coding sequence?: Y Chromosome: V; Localization: V L, 7 cM from CEN Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: RPR1 Gene name: RNA component of nuclear RNase P Contains complete coding sequence?: Y Chromosome: V Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: MMS21 Contains complete coding sequence?: Y Chromosome: V Strain: AB972 (ATCC 76269) Type of DNA: genomic Insert end: MboI Insert end: MboI Insert size (kb): 38.0 Cross references: DNA Seq. Acc.: U18530 Nucleotides ? of the insert correspond to nucleotides 1-38086 of U18530. DNA Seq. Acc.: U18779 Nucleotides ? of the insert correspond to nucleotides 31670-36872 of U18779. Insert lengths(kb): 38.0 Gene product: Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex CUP5 |
| Insert Size (kb)： | 38.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: frozen, uncharacterized |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |