微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pCYM01 |
|---|---|
| 商品货号： | TS137326 |
| Designations： | pCYM01 |
| Depositors： | JH Camonis |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.7449998855590820 Vector: pCYM01 (plasmid) Promoters: Promoter SV40 early Construction: pCYM1 (ATCC 37822), pMC1403 Marker(s):URA3,ampR Construct size (kb): 8.744999885559082 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: SV40 early replicon: pMB1, 2 micron terminator: SV40 |
| Applications： | YE-type (episomal) shuttle vector expression vector reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/PvuII--4.8, 2.6, 0.5, 0.46; EcoRI--8.4; SmaI--8.4. Shuttle expression vector which includes a reporter group. Expresses beta-galactosidase activity in E. coli, S. cerevisiae, and mouse cells, and thus can be used to calibrate transformation efficiency. Also permits blue/white detection in E. coli. The EcoRI, SmaI, and BamHI sites are 3 to the unique available in-phase initiation codon under control of the SV40 early promoter. Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403 into pCYM1 cut with BamHI (nt 427), linkered to add an EcoRI site, and cut with BalI (nt 702). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907 |
| Shipped： | freeze-dried |